Coding

Part:BBa_K4701224:Design

Designed by: Henri Sundquist   Group: iGEM23_Aalto-Helsinki   (2023-08-03)


1,2-dihydroxy-3,5-cyclohexadiene-1,4-dicarboxylate dehydrogenase (tphB)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimization for Pseudomonas putida was done with a tool provided by IDT. BioBrick restriction sites were removed manually. The design was heavily dependent on the design of a synthetic operon. See the details here, and on our wiki.

Source

The original nucleotide sequence for tphBII was acquired from GenBank: AB238679